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Springer Editions. These structural differences can significantly affect FITC-dextran delivery due to even after microporation [30]. The calculated dextran molecular weights are corroborated by those tabulated data by the manufacturer with a relative error lower than 10%, being the exception dextrans 580 and 2000kDa with the highest relative error. WebAmines per 70KDa dextran Theeffectofchargerepulsiononthe hydrodynamicradiuscanbeseenwithRh increaseswithchargedensity.Charge repulsioncausesthehydrodynamicradius toincrease.Withincreasingcharge,the dextranprogressivelyunfolded.Evena lowlevelofaminationcausesthepolymer tounfold. A simple and rapid method for evaluation of MarkHouwinkSaturada constants of linear random coil polysaccharides using molecular weight and intrinsic viscosity determined by high performance size exclusin chromatography: application to guar galactomannan. Kasaai, M. R.; Arul, J.; Charlet, G. (2000). By studying the molecular weights of various solutions of polymer, Solomon-Ciuta arrived at the formula which allowed the calculation of the intrinsic viscosity of polymer solutions by a single viscosity determination. Journal of Pharmaceutical and Biomedical Analysis, 41, 1260-1267. After 24h, the cumulative permeation of FITC-dextran M.W. Learn More. sharing sensitive information, make sure youre on a federal of different origins. Liu Zonghua; Yanpeng Jiao; Yifei Wang; Changren Zhou; Ziyong Zhang (2008). Chromatographic characterization of polymers: Hyphenated and multidimensional techniques. Thermo Fisher Scientific. Article Figure2 shows TEWL values measured before and immediately following the treatment of human cadaver skin with the microporation device. An undergraduate physical chemistry experiment. Polyelectrolytes as secondary calibration standards for aqueous SEC. Format). Table1 shows TEWL values measured immediately after the microporation of human and pig skins. The intrinsic viscosity can be easily calculated by the Solomon-Ciuta single-point equation [44, 45]. WebHydrodynamic radius = variable size of an analyte with specific MW based on dynamical properties. (1969). Bose, A.; Rollings, J. E.; Caruthers, J. M.; Okos, M. R.; Tsao, T. G. (1982). Die Makromolekulare Chemie, 148(1), 229-238. Intrinsic viscosity determination offers a simple and useful method that requires low cost equipment and yields useful information on soluble macromolecules. There is a formula that used to calculate the radius (in Angstrom) from the molar mass (in g/mol) of dextran, a=0.33*(MM^0.46) where, a and MM are Data File Dextran. The human and pig skin samples were placed in the receiver chamber of the Transdermal diffusion cell system with the SC facing up and the donor chamber was fixed in place. dextran hydrodynamic radius. For a given polymer-solvent pair, the theta condition is satisfied at a certain temperature, called the theta () temperature or theta point. Viscosity, sedimentation, and light-scattering properties of fraction of an acidhydrolyzed dextran. Challenges for the modern analytical ultracentrifuge analysis of polysaccharides. International Journal of Pharmaceutics, 277, 99-104. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. FITC-dextran molecular weights; 4, 10, and 20kDa molecular weight were purchased from Sigma (St. Louis, MO, USA). The Mark-Houwink a constant is close to 0.5 in poor solvents. Transdermal delivery is of great importance for the effective delivery of bioactive or therapeutic agents into a body. WebFITC Products. Careers. Rissmann R, Oudshoorn MHM, Hennink WE, Ponec M, Bouwstra JA. Citation-(RIS Bahary, W.S. Microporation was performed 2, 5, and 10 times on each sample. Many works are aimed at determining molecular weights [1, 7, 26], hydrodynamic radius, among other parameters for characterizing the physics and chemistry properties of dextran in solution. Immediately after 2, 5, and 10 times microporations, 97.1432.39, 80.5715.51, and 82.3916.49m sizes of micropores were formed on the skin, respectively. WebDextran 40 Pharmaceutical Quality. Appl Biochem Biotechnol. Colloid Polym. Advances in Cosmetic Surgery. Macromolecules. Food Sci Biotechnol. Viet, D.; Beck-Candanedo, S.; Gray D. G. (2008). Boudry I, Blanck O, Cruz C, Blanck M, Vallet V, Bazire A, Capt A, Josse D, Lallement G. Percutaneous penetration and absorption of parathion using human and pig skin models in vitro and human skin grafted onto nude mouse skin model in vivo. 2016;47:15964. Human cadaver skins (fresh human full-thickness skin disc, 12~20mm) were supplied by Biopredic International (Saint-Grgoire, France). 2015;23(3):2239. The Mark-Houwink a constant is close to 0.75 (or higher) for these good solvents. DOI: 10.12691/jpbpc-1-1-3, Received September 18, 2013; Revised October 23, 2013; Accepted November 22, 2013. Strategies to improve the potential of transdermal devices by enhancing the skin permeation of therapeutic entities. Webprotein hydrodynamic radius was observed with increasing protein concentration (in the range 0.25-2 mM) in both the absence and presence of 8 M urea. 43-1, 115-147. Journal of Chemical Education, 80(9), 1036-1038. The Transdermal diffusion cell system was protected from light. Bookshelf Increased epidermal laser fluence through simultaneous ultrasonic microporation. Chem. Hydrodynamic radii were measured using Dynamic Light Scattering (DLS) in a Malvern Zetasizer NanoZS DLS (Malvern Instruments, Malvern, UK) at high scattering angle (173) to minimise the effects of dust and supramolecular contamination (NIBS, 173). (1998). Soc., 16, 3A, 426-433. 10K, and c M.W. Journal of Physical Chemistry. Covis, Rudy; Ladaviere, Catherine; Desbrieres, Jacques; Marie, Emmanuelle; Durand, Alain. Superior prebiotic and physicochemical properties of novel dextran from Weissella cibaria JAG8 for potential food applications. Middelkoop E, Bogaerdt AJ, Lamme EN, Hoekstra MJ, Brandsma K, Ulrich MMW. Solution properties of branched dextran. 2009;6(4):34354. For data requests, please contact the authors. 4K and M.W. These hydrodynamic parameters show that these polysaccharides behave as a compact rigid sphere and contract by the increase of temperature (RH decreases) for the Mw range from 8.8 to 200kDa. (2000). Int J Pharm Investig. These modifications include degradation of hemidesmosomes attached to the dermis, degradation of desmosomes linked to adjacent cells, contraction of intracellular tonofilament, and lamellipodia formation. Anal. J Appl Toxicol. For 40 kDa molecules, the hydrodynamic diameter dh ~8 nm is much smaller than the collagen mesh size [ 33, 41 ]. ; Bos, G.W. Skin barrier disruption by acetone: observations in a hairless mouse skin model. The hydrodynamic radius is important in predicting transretinal penetration Carroll PJ. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was ; Garca de , J. H&E stained images were analyzed using Image J software. This result could reflect the presence of some aggregated species within the lysozyme sample solution. WebFluorescein isothiocyanatedextran average mol wt 60,000-76,000; CAS Number: 60842-46-8; Synonyms: FITCDextran; find Sigma-Aldrich-FD70S MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich Molecular weight (Mv) and hydrodynamic properties can be calculated from there. Armstrong, J. K.; Wenby, R. B.; Meiselman, H. J.; Fisher, T. C. (2004). To overcome these limitations, microporation devices that act as therapeutic vehicles for delivering drug molecules across the skin barriers have been developed. 8600 Rockville Pike Where f0 is the frictional coefficient of a sphere having the same hydrodynamic (hydrated or solvated) volume V as the particle. Keratinocytes adjacent to the wound site induced by the microporation in the epidermis undergo a series of modifications that allow their proliferation and migration to the wound. Intrinsic viscosity molecular weight relationship. II. This device can generate microchannels from the SC through to the outer dermis and allow transdermal delivery of active agents. The relation between Mw and the intrinsic viscosity is given by Mark-Houwink equation. Lpez Martnez, M. C.; Daz Baos, F. G.; Ortega Retuerta, A.; Garca de , J. Search Journal of American Chemical Society, 76 (20), 5041-5052. TEWL measurements were performed before and 30min after microporation. Molecular weight (M v) and hydrodynamic properties can be calculated from there. Correspondence to (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity. Lim SB, Tingirikari JMR, Seo JS, Li L, Shim S, Seo JH, Han NS. The optical image and histological analysis confirmed that the micropores on the skin were recovered over time. Transdermal drug delivery is an attractive alternative that addresses the limitations of oral and parenteral routes of drug administration; it enables controlled release and long-term systemic drug delivery through the skin, avoids hepatic first-pass effect, avoids gastrointestinal drug degradation, reduces discomfort and trauma resulting from hypodermic injections, and prevents safety hazards associated with dangerous medical waste from needles [1, 2]. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. TEWL measurements were taken to assess the integrity of the skin barrier and the extent of SC disruption. 2012;3(1):208. Hydrodynamic cavitation (HC), a common phenomenon that can produce local excessive temperature, high pressure and large amount of energy. Hrabchak C, Flynn L, Woodhouse KA. The above data are not useful because it can not be compared with those obtained in this work. Dextran Fractions. (2008). HHS Vulnerability Disclosure, Help Recent Pat. Radiofrequency is a well-known medical technique with expanded application in electrosurgery for laparoscopic medical procedures and in dermatology, for sebaceous glands ablation in acne treatment [12, 13], and treatment of photoaging skin symptoms, including wrinkles and hyperpigmentation [14]. Google Scholar. Antoniou, E.; Buitrago, C. F.; Tsianou, M.; Alexandridis, P. (2010). Int J Food Sci Nutr. Carbohydrate Polymers, 38, 267-271. After 24h the micropores were either closed or reduced further to smaller sizes, 37.5020.41, 50.5718.07, and 58.5814.99m, for 2, 5, and 10 times microporation, respectively. Biophysical Journal, 87, 4259-4270. Ioan, C.E. The great deviation at 2000kDa occurs due to the large hyperbranched structure of the macromolecule, generating a change in its conformation and, therefore, a change in the way it flows [73]. These vibrations heat the skin tissue, triggering water vapor and cell ablation, forming microchannels from the SC through to the outer dermis. WebThe invention relates to novel fluorescent dyes with multiple negatively charged groups in their ionized form which are 9-aminoacridines or 1-aminopyrene shaving of one of the fol An official website of the United States government. Kasaai, Mohammad R. (2007). In Vitro Evaluation of Postbiotics Produced from Bacterial Isolates Obtained from Rainbow Trout and Nile Tilapia against the Pathogens, Dextran-Mimetic Quantum Dots for Multimodal Macrophage Imaging, Isolation of lactic acid bacteria starters from, Characterization of Antibacterial Cell-Free Supernatant from Oral Care Probiotic. A novel high dextran yielding Weissella cibaria JAG8 for cereal food application. At 25C both values of a and kM-H are within the range reported by [3, 8, 16]. Part of The Mark-Houwink parameters is calculated molecular weights range from 8.8 to 200kDa. Teixeira CG, Fusieger A, Milio GL, Martins E, Drider D, Nero LA, de Carvalho AF. (2013). Journal Argentine Chemical Society 100 (2013) 25-34. Unauthorized use of these marks is strictly prohibited. For rigid macromolecules, it is also conventional to combine a solution property with the volume of the particle itself, or with a quantity directly derived from it [54, 55, 56]. ; Yevlampieva N.P. The solution viscosity () is related to the solvent viscosity (o), resulting in the relative viscosity. Polymolecularity and polydispersity in Molecular weight determinations. The a value is higher than calculated in this work, because these calculations were performed with number average molecular weight (Mn) which rearranges the value of a and forces him to enter into unbranched or linear molecules (a > 0.5), on the other hand, if molecular weights (Mw) ware used to value would be less than 0.5. Schematic illustration of transdermal delivery after the application of the microporation device. Treatment of acne scars by fractional bipolar radiofrequency energy. (1999). The values of Mark-Houwink parameters universalized for the range of working temperatures are the following: 0.4267 for a*, and 0.1455 cm3/g for k*M-H, with 2 of 0.9399. This is because the macromolecule hydrodynamic radius changes the solution type and temperature via change in their chain exibility. The effectiveness of the RF microporation technique in transdermal drug delivery of macromolecules and hydrophilic agents such as peptides, hormones, and vaccines has been demonstrated extensively in previous studies [16,17,18]. In this work, the human cadaver skin was treated to confirm the skin regeneration after the application of the microporation device. 2023 BioMed Central Ltd unless otherwise stated. Microneedles and their applications. (1954). TEWL values before and immediately after microporation on human cadaver skin according to the number of times microproration was done (n=3). Article Similar results are obtained in references [35, 36, 37], using a system of dextran / ethylene glycol, and dextran/dimethylsulfoxide, respectively. Federal government websites often end in .gov or .mil. Carbohydrate Polymers, 79, 380-390. The exponent a is a function of polymer geometry, and varies from 0.5 to 2 and exponent is dimensionless. (1999). Stenekes, R.J.H. With FITC-dextran 20 K, cumulative Senti, F. R.; Hellman, N. N.; Ludwig, G.; Babcock, E.; Tobin, R.; Class, C. A., (1955). Table 1 shows some values of dextrans Mark-Houwink parameters, from literature consulted, where all data are based on the logarithmic plot of intrinsic viscosity [] and molecular weight Mw. One day before the experiment, the skin was stabilized in the skin culture medium. Pharm Res. Synthesis of water-soluble and water-insoluble amphiphilic derivatives of dextran in organic medium. Eng. J. Pharm Res. Thus, it is accepted that, for every flexible-chain polymer in a (ideal) solvent, there is a universal value of 0 = 2.1-2.5x1023mol-1 [52]. Transdermal drug delivery system for non-steroidal anti inflammatory drugs: a review. eCollection 2015. osmotic pressure or light scattering). Radiofrequency ablation of malignant liver tumors. The temperature increment can induce a macromolecule compaction (decreases in RH and []), generating by this way, a greater difficult to flowing due at requires an increase of energy consumption (Eavf<). Fractional thermolysis by bipolar radiofrequency facilitates cutaneous delivery of peptide and siRNA with minor loss of barrier function. Quantitative data are presented as the meanstandard deviation, and comparisons were carried out using one-way ANOVA (Systat Software Inc., Chicago, IL, USA). (2003). Naik A, Kalia YN, Guy RH. The .gov means its official. These results could be attributed to the differences in the in vivo and in vitro skin nutrients supply; with in vivo studies, the skin tissue is supplied nutrients directly from the blood vessels, but the human cadaver skin is supplied from the media, therefore, the skin tissue regeneration efficiency is low [26,27,28].